The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. ® reader to the computer. SpectraMax® M5 Multi-Mode Microplate Reader (cat. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Flexible for 6-well to 384-well microplates and cuvettes. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. 313 reference gain check fail. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Wide range of applications. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Has anyone had problems with the built-in computer of the Spectramax ID3. 1–0. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. 73. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. In brief, RAW 264. 87. Add to Cart. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Most papers they cultured bacteria to OD600>0. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Step 2. Figure 1. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Download PDF. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. When a feature or capability applies to only certain readers, this exception is noted. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Cuvette port functionality can also be controlled using SoftMax Pro Software. 0 out of 5 $ 3,799. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. To block unwanted residual excitation light and minimize background interference, a 570 nm. Lift up the lid over the chamber to insert or remove a cuvette. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. . Before you Begin Materials Required but Not Provided • Fresh, anhydrous. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. 4. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. Amino acids assay. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. The SpectraMax L Microplate Reader was set up for endpoint reading with an inte-gration time of 1 second or 0. All associated StakMax® stackers. Table of Contents. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. Microplate Readers include the M3, M4, M5, and M5e models. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. 7 cells were co-transfected with 0. SpectraMax® Microplate Readers and SoftMax® Pro Software. View the Molecular Devices website. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 7°C (gradient heating design). AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. 1. To quantify the A1AT in rat serum, standard curves were drawn for nA1AT, rA1AT-WT and rA1AT mutants, respectively. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Plate Shaking. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 2. Call 516-939-0888 to check. The absorbance values at 450 and 570 nm were determined using the SpectraMax M5 microplate reader (Molecular Devices, Silicon Valley, CA, United States). 6 0. After 24 h drug treatment, cells were lysed by RIPA buffer (Life Technologies Inc, US). The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). While they play an important role in generating data from a wide range of. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Do not touch the clear surfaces of the cuvette. The cuvette must be in place when you read the plate. 303 unable to cal dark current. The chamber contains springs that position the cuvette in the proper alignment for a read. Italic and boldface type are used for emphasis. Figure 1. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. 2. 0 μL) instead of the sample solution was applied as control group, and. Molecular Devices. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. analysis, controls the SpectraMax Multi-Mode Microplate Readers. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. spectramax m5 multi. Other technologies such as imaging. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . For 40 years, we have partnered with scientists to help them achieve landmark discoveries. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. Microplate Readers Biotek Synergy H1 Hybrid Microplate. Temperature variation ± 0. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Make sure the microplate is flat against. Table of Contents. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. l. Validate the optical performance of your absorbance . Figure 1. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The percentage of viable cells for each treatment group was calculated by adjusting the untreated control group to 100%. Keywords. Go to Product Details. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Sale price $16,495. SpectraMax® M4. free tracer decreases, resulting in. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Wide range of applications. Temperature control range from Ambient +4º to 50ºC. The CDWIA was performed on each. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 4. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. SpectraMax Plus 384 (Molecular Devices) [4 units] UV and visible absorbance monochromator-based microplate reader SpectraMax M5 (Molecular Devices) [2 units] multi-label monochromator and filter-based microplate reader for UV and visible absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence. View the Molecular Devices website. com. 0 out of 5 $ 16,499. 306 plate air cal. -Wide range of applications -Five preferred detection modes -Maximum signal with lowest background The dual monochromator optics allow the widest range of. Protocol Name Version 6. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Ltd. The advantage of using labeled probes is detection specificity. Standard features include a. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The assay window spanned four orders of magnitude. 2. SpectraMax® Aquamax. When the user applies a set temperature of 37°C, the top is heated to 37. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Add to Cart. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. 7°C (gradient heating design). Key applications: ELISA, nucleic acid, endotoxin testing. 00. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. All associated StakMax® stackers. Microplate Reader Shelf. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Turn on the microplate reader. Published Dec 16, 2020 | Updated Dec 16, 2020. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Turn on the microplate reader. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. As. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. Molecular Devices SpectraMax® series microplate readers. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. The readings with excitation at 540 nm and emission at 600 nm represented the amount of phosphorylated IκB-α in the cells. Microplate Readers include the M3, M4, M5, and M5e models. book Page 7 Wednesday, July 7, 2010 2:09 PM. . Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The acceptance. Our absorbance plate readers feature our PathCheck Sensor. 87. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Or, with the reader specifications noted from the Instrument Description section of your reader's. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). This reader's dual monochromators for. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. 315 can't find zero order. Methods Preparation of reagents and standards. 96-384. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. 67. Plate chamber design of the SpectraMax category 1 microplate readers. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. These lab workhorses are used in drug discovery, research, bioassay validation, and. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 5. SpectraMax® Aquamax. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. , Sunnyvale, CA) using the bottom well-scan mode where nine readings. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Molecular Devices. FlexStation™ I. The assay applies fluorescence polarization to quantify Fc-containing IgG. Maximum signal with lowest background. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Ltd. Examples: “Press carefully to engage,” “ Do not. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Table of Contents. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). Western blotting analysis. Western blotting assay. Pick the best speed, sensitivity, and price for your needs. SpectraMax iD5 Multi-Mode Microplate Reader. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. The integration time suggested is 5-10 sec. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. metrically at 540 nm using a microplate reader (SpectraMax 384 Plus, Molecular Devices Corp. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Fluorescent signals were measured using the SpectraMax/M5 fluorescence spectrophotometer plate reader (Molecular Devices Co. 0 out of 5 $ 24,695. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. ZERO BIAS - scores, article reviews, protocol conditions and moreThe absorbance was measured at 450–650 nm using a SpectraMAX M5 microplate reader (Molecular Devices). 3. SpectraMax® ABS. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). 1. Designed to provide the simplicity, flexibility and power required for advanced data analysis. Cuvette port functionality can also be controlled using SoftMax Pro Software. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe reader’s stated range. Small, unbound molecules rotate rapidly in. Microplate Readers Biotek Epoch Microplate. Microplate reader settings. The day before assay, assay buffer was prepared as described in the product technical manual. Then, the fluorescence change rates (F 0 − F)/F 0 were calculated, and the selectivity histogram. 0 . When a feature or capability applies to only certain readers, this exception is noted. RAW 264. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. Compatible automation solutions for SpectraMax Readers. begin reading microplates with seven mouse clicks. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The system. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. used. 304 signal level saturation. p. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). B: Selection of Excitation Wavelength. 6% for the on-board optics (monochromators). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Fluorescence was measured (Ex 530/Em 590) in a SpectraMax M5 microplate reader. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. E Models Only) 69. We would like to show you a description here but the site won’t allow us. FlexStation™ II. . SpectraMax Lmicroplate luminometer. com 13. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. Learn how you can utilize microplate readers to drive landmark discoveries. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. 5 Dual-luciferase reporter assay. TNF standard curve. 7°C and the bottom to 36. 314 low lamp level warning. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Table of Contents. Shop now. . Thank you!. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. 3 1. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Page 1 of 8 . B: Selection of Excitation Wavelength. CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. Wide range of applications The VersaMax reader covers. Compact and tunable absorbance microplate readers DATA SHEET. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Download PDF. The PBS solution (10. TR-FRET assay. 1 second. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Microplate Readers include the M3, M4, M5, and M5e models. Here the delta mP was 336, and Z’ factor was 0. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. These robust readers have been placed in labs from Antarctica to. M3 M4 M5 M5e LM1 v<nnn>. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. PMT sensitivity was set to AutoRange with a target calibration wavelength of 470 nm, and the plate was dark adapted for 1 minute prior to reading. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. For a higher . An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. 5 12. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Uncontrolled Copy for Training and Reference Purposes Only . Optical absorbance. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Microplate Readers include the M3, M4,. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Introduction. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 00 USD. 67. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. TR-FRET assay principle. 2. RAW 264. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. The dual monochromator optics allow the widest range of applications to be utilized for bior. FlexStation™ II. 00 USD. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Microplate Readers. 2. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-,. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. Page 28 Chapter 5: Read Modes and Read Types 1. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 72 MB. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Cables. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Three independent experiments were performed. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 3 mg. Lower limits of detection for ATP determined using the ATPlite . With optimized reagents, option to operate the readers. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Expanded wavelength coverage. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 0 OD for earlier models. The following document is. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. 0 2. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance,. Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). The cells were then treated to induce apoptosis.